Study BMB 464 Exam 1 Flash Cards

 
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BMB 464 Exam 1

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Cann, Stoneking, and Wilson (1987)
-proposed all humans evolved from 1 common ancestor: "Mitochondrial Eve"
Mullis (1983)
-created PCR
Timeline
Late 1960’s: Restriction Endonucleases

1972: First recombinant DNA molecules made by ligase

1975: Hybridization of small probes to DNA by Southern

1976: First eukaryotic gene cloned by Maniatis

1977: sequencing DNA

1978: DNA from Adenovirus found to be discontinuous, “exon” and “intron” were coined

1980s: Use of polymorphisms to create maps

1982: Human insulin first GE drug from Genetech

1987: homo sapiens evolved from common African ancestor

1988: First genetically engineered mouse patented in Harvard Medical School
Maniatis (1976)
-cloned first eukaryotic gene, rabbit globulin
Watson & Crick (1953)
-propose double helix structure of DNA
‐beginning of “molecular biology”
-Wilkins and Franklin generated data to decipher molecular structure
Chargaff (1950)
showed A=T & C=G
Avery, MacLeod, McCarty (1944)
-showed Griffith's "transforming principle"was DNA
-mice treated w/ DNase--> lived
-mice treated w/ protease--> died
-demonstrated that genetic info in Pneumococcuswas in DNA
Miescher (1869)
-coins the term “nuclein”
-isolated nuclei from pus cells
Gilbert and Sanger (1977)
-each devised methods to determine DNA nt sequence
-shared Chem Nobel Prize in 1980
Southern (1975)
-realized he could use agarose to detect sequence-specific DNA pieces
Berg (1972)
-produced 1st hybrid DNA made by ligase (from different organisms)
Smith (1960-70)
-discovered site-specific restriction enzymes
Kornberg (1956)
-isolated DNA polymerase
-first to synthesize DNA in a test tube
Hershey and Chase
-provided support that DNA (not protein) is hereditary material of cell
Grifftih
-showed info could be transferred b/w different strains of bacteria

-rough strain--> mice lived
-smooth strain--> mice died
-heated smooth--> mice lived
-heated smooth + rough --> mice died (transformation)
first GE drug
Human insulin (1982)
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