Study New BMB 401H Chap 12 Flash Cards

 
Pile Management Card
New BMB 401H Chap 12

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freeze fracture
-freeze membrane to -196C
-split with cold microtome knife
-metallic replica made
Sendair virus experiment
-yielded hybrid heterokaryon
-label mouse proteins w/ green antibodies
-label human w/ red
-after 40 min at 37C, proteins intermingled
-at 15C, slowed the process
fluid mosaic model
-rotational/lateral mvt
-proteins= icebergs
peripheral proteins
-removed by mild procedures
integral proteins
-tightly bound
-removed only by detergents
membrane phase transition
-below melting temp, very close
-10-40C
dynamic simulation
-viscosity increases closer to head group
-tails interdigitated
-order water around head group
bleaching
-flourescent markers
-bleach
-recovery rate
lateral diffusion
-fast
transverse diffusion (flip-flop)
-slow
liposome
-stable
-used for drug delivery
-fuse with cell membrane and dump contents in
-permeable to water
cholesterol
-sterol
-animal plasma membrane
-membrane fluidity
gangliosides
-cell surface membranes
-carbs are receptors
-cell/cell recognition
cerebrosides
-neurons
spingomyelins
-choline, ethanolamine group
-nervous tissue
-insulate axons
sphingolipids
-spingomyelins, cerebrosides, gangliosides
plasmalogens
-ether linked
-serine, choline, ethanolamine most common head groups
triacyglycerol
-3 fatty acids to glycerol
-major energy reserve
-good insulators
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