Study New BMB 401H Chap 9 Flash Cards

 
Pile Management Card
New BMB 401H Chap 9

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Kuru
-Papau New Guinea
-cannibalism
bovine spongiform encephalopathy
-mad cow disease
scrapies
-sheep scrape their wool on fence
nucleoplasmins
-acidic nuclear proteins
-required for in vivo assembly of nucleosomes
Hsp90
-folds proteins involved in signal transduction
Hsp70
-protein unfolding increased at high temps
-bind to newly synthesized proteins off ribosome
-help unfold proteins if they have to traverse a membrane
dithiothreitol
-oxidizes BPTI
BPTI
-bovine prancreatic trypsin inhibitor
-fully inactive if reduced
-3 disulfide bonds
-oxidized by dithiothreitol
circular dichroism
-measure amt of helical structure
-proteins absorb in UV region due to aromatic compounds
intrinsic fluorscence of Rnase A
-used to measure denaturing of protein
Janus
-similar to GB1 in structure
-shows not all aas important in protein structure
GB1
-bacterial protein that binds to IgG
-change 50% of proteins to turn into Janus (Rop protein)
mercaptoethanol
-reduces disulfide bonds
chaotropic salt
-disrupts the three dimensional structure in macromolecules
-interfere with stabilizing intra-molecular interactions mediated by non-covalent forces such as hydrogen bonds, van der Waals forces, and hydrophobic effects.
urea
-chaotropic salt
native vs. denatured energy chnage
-small
-diff b/w entropy and enthalpy
entropy
-disorder
-favors denatured state
enthalpy
-energy of non-covalent bonds (H bonds, ionic bonds, etc.)
-favored in native state
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