Study BMB 400 Chapter 10 Flash Cards

 
Pile Management Card
BMB 400 Chapter 10

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HO endonuclease
introduces double-strand DNA break in MAT
mitosis vs. meiosis
Rad51 expresses both mitotically and meiotically, but Dmc1 is expressed in only meiosis
Rad51 and Dmc1
are bacterial RecA homolog in eukaryote and play a role for meiotic recombination
MRX complex
(Mre11, Rad50 and Xrs2) replaces SPO11 protein on DNA to produce 3’ single stranded DNA (>1 kb) for homologous recombination
Tyrosine side chain
attacks the phosphate backbone to cut DNA, and generate covalent complex between protein and DNA
SPO11:
introduces double-strand breaks, cut-sites are located most commonly in chromosomal regions that are not tightly packed with nucleosomes such as promoters (hotspots for recombination)
RuvC (dimer) is
DNA endonuclease to resolve Holiday junction
RuvC cleaves DNA with modest sequence specificity. The consensus sequence is found frequently in DNA, but the sequence specificity ensures at least some branch migration occurs before resolution
RuvB
ATP-dependent hexameric DNA helicase for branch migration
RuvA
Holiday junction specific DNA binding protein, recruiting RuvB proteins
RecA Homologs Are Present in All Organisms
RecA: eubacteria
RadA: archaeal
Rad51 and Dmc1: eukaryote
UvsX: T4 phage
These homologs form similar filament structures
None (E. coli)
introduction of DSB
-breaks introduced via DNA damage or failure of rep fork
RuvC (E. coli)
resolution of Holiday junctions
RuvAB (E. coli)
Holiday junction recognition and branch migration
RecBCD and RecFOR (E. coli)
assembly of strand-exchange proteins
RecBCD helicase/nuclease (E. coli)
processing DNA breaks to generate single strands for invasion
RecA (E. coli)
pairing homologous DNA and strand invasion
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