Study BMB 400 Chapter 8 Flash Cards
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Pile Management Card
BMB 400 Chapter 8
BMB 400 Chapter 8
| DNA helicases |
|
-Heximeric proteins -ATP -Ring shaped protein complexes encircle the DNA strand (increase processivity) -moves directionally |
| 5’ exonuclease |
| -removes final RNA that removed from 5' end |
| RNA primers are removed by |
|
1) RNase H plus 5’ exonuclease or 2) 5’->3’ exonuclease activity of DNA pol I DNA pol I fills gap and DNA ligase repairs a nick |
| RNA primer |
| (5~10 nt long) |
| Primase |
|
-RNA primer (5~10 nt long) -does not require specific DNA sequences to initiate syntheis -activated when associated with DNA helicase -removed by RNase H plus 5’ or 5’->3’ exonuclease activity of DNA pol I |
| Processivity |
| -average number of nucleotides added each time the enzyme binds a primer:template junction |
| Kinetic selectivity |
| -Incorrect nucleotide incorporation is as much as 10,000-fold slower than incorporation when base pairing correct. Error rate ~10-5 |
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