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| Cann, Stoneking, and Wilson (1987) |
-proposed all humans evolved from 1 common ancestor: "Mitochondrial Eve" |
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mcs5109 Mon, 28 Sep 2009 00:51:03 GMT |
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| Mullis (1983) |
-created PCR |
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mcs5109 Mon, 28 Sep 2009 00:51:03 GMT |
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| Gilbert and Sanger (1977) |
-each devised methods to determine DNA nt sequence
-shared Chem Nobel Prize in 1980 |
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mcs5109 Mon, 28 Sep 2009 00:44:19 GMT |
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| Southern (1975) |
-realized he could use agarose to detect sequence-specific DNA pieces |
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mcs5109 Mon, 28 Sep 2009 00:44:19 GMT |
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| Berg (1972) |
-produced 1st hybrid DNA made by ligase (from different organisms) |
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mcs5109 Mon, 28 Sep 2009 00:44:19 GMT |
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| Smith (1960-70) |
-discovered site-specific restriction enzymes |
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mcs5109 Mon, 28 Sep 2009 00:40:18 GMT |
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| Kornberg (1956) |
-isolated DNA polymerase
-first to synthesize DNA in a test tube |
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mcs5109 Mon, 28 Sep 2009 00:40:18 GMT |
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| Hershey and Chase |
-provided support that DNA (not protein) is hereditary material of cell |
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mcs5109 Mon, 28 Sep 2009 00:35:43 GMT |
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| Grifftih |
-showed info could be transferred b/w different strains of bacteria
-rough strain--> mice lived
-smooth strain--> mice died
-heated smooth--> mice lived
-heated smooth + rough --> mice died (transformation) |
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mcs5109 Mon, 28 Sep 2009 00:32:08 GMT |
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| first GE drug |
Human insulin (1982) |
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mcs5109 Fri, 25 Sep 2009 18:55:45 GMT |
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| Maniatis (1976) |
-cloned first eukaryotic gene, rabbit globulin |
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mcs5109 Mon, 28 Sep 2009 00:47:07 GMT |
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| Timeline |
Late 1960’s: Restriction Endonucleases
1972: First recombinant DNA molecules made by ligase
1975: Hybridization of small probes to DNA by Southern
1976: First eukaryotic gene cloned by Maniatis
1977: sequencing DNA
1978: DNA from Adenovirus found to be discontinuous, “exon” and “intron” were coined
1980s: Use of polymorphisms to create maps
1982: Human insulin first GE drug from Genetech
1987: homo sapiens evolved from common African ancestor
1988: First genetically engineered mouse patented in Harvard Medical School |
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mcs5109 Mon, 28 Sep 2009 00:49:47 GMT |
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| Watson & Crick (1953) |
-propose double helix structure of DNA
‐beginning of “molecular biology”
-Wilkins and Franklin generated data to decipher molecular structure |
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mcs5109 Mon, 28 Sep 2009 00:46:05 GMT |
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| Chargaff (1950) |
showed A=T & C=G |
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mcs5109 Mon, 28 Sep 2009 00:45:19 GMT |
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| Avery, MacLeod, McCarty (1944) |
-showed Griffith's "transforming principle"was DNA
-mice treated w/ DNase--> lived
-mice treated w/ protease--> died
-demonstrated that genetic info in Pneumococcuswas in DNA |
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mcs5109 Mon, 28 Sep 2009 00:45:00 GMT |
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| Miescher (1869) |
-coins the term “nuclein”
-isolated nuclei from pus cells |
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mcs5109 Mon, 28 Sep 2009 00:44:40 GMT |
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