| front |
back |
revisions |
lasted changed by |
history |
| freeze fracture |
-freeze membrane to -196C
-split with cold microtome knife
-metallic replica made |
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mcs5109 Thu, 13 Nov 2008 07:02:15 GMT |
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| Sendair virus experiment |
-yielded hybrid heterokaryon
-label mouse proteins w/ green antibodies
-label human w/ red
-after 40 min at 37C, proteins intermingled
-at 15C, slowed the process |
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mcs5109 Thu, 13 Nov 2008 07:02:15 GMT |
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| fluid mosaic model |
-rotational/lateral mvt
-proteins= icebergs |
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mcs5109 Thu, 13 Nov 2008 07:02:15 GMT |
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| peripheral proteins |
-removed by mild procedures |
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mcs5109 Thu, 13 Nov 2008 06:56:20 GMT |
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| integral proteins |
-tightly bound
-removed only by detergents |
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mcs5109 Thu, 13 Nov 2008 06:56:20 GMT |
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| membrane phase transition |
-below melting temp, very close
-10-40C |
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mcs5109 Thu, 13 Nov 2008 06:56:20 GMT |
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| dynamic simulation |
-viscosity increases closer to head group
-tails interdigitated
-order water around head group |
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mcs5109 Thu, 13 Nov 2008 06:56:20 GMT |
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| bleaching |
-flourescent markers
-bleach
-recovery rate |
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mcs5109 Thu, 13 Nov 2008 06:51:07 GMT |
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| lateral diffusion |
-fast |
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mcs5109 Thu, 13 Nov 2008 06:51:07 GMT |
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| transverse diffusion (flip-flop) |
-slow |
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mcs5109 Thu, 13 Nov 2008 06:51:07 GMT |
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| liposome |
-stable
-used for drug delivery
-fuse with cell membrane and dump contents in
-permeable to water |
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mcs5109 Thu, 13 Nov 2008 06:51:07 GMT |
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| cholesterol |
-sterol
-animal plasma membrane
-membrane fluidity |
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mcs5109 Thu, 13 Nov 2008 06:41:02 GMT |
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| gangliosides |
-cell surface membranes
-carbs are receptors
-cell/cell recognition |
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mcs5109 Thu, 13 Nov 2008 06:41:02 GMT |
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| cerebrosides |
-neurons |
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mcs5109 Thu, 13 Nov 2008 06:41:02 GMT |
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| spingomyelins |
-choline, ethanolamine group
-nervous tissue
-insulate axons |
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mcs5109 Thu, 13 Nov 2008 06:41:02 GMT |
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| sphingolipids |
-spingomyelins, cerebrosides, gangliosides |
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mcs5109 Thu, 13 Nov 2008 06:32:32 GMT |
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| plasmalogens |
-ether linked
-serine, choline, ethanolamine most common head groups |
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mcs5109 Thu, 13 Nov 2008 06:31:15 GMT |
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| triacyglycerol |
-3 fatty acids to glycerol
-major energy reserve
-good insulators |
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mcs5109 Thu, 13 Nov 2008 06:31:15 GMT |
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