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history |
| Kuru |
-Papau New Guinea
-cannibalism |
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mcs5109 Wed, 12 Nov 2008 19:06:47 GMT |
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| bovine spongiform encephalopathy |
-mad cow disease |
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mcs5109 Wed, 12 Nov 2008 19:06:47 GMT |
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| scrapies |
-sheep scrape their wool on fence |
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mcs5109 Wed, 12 Nov 2008 19:06:47 GMT |
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| nucleoplasmins |
-acidic nuclear proteins
-required for in vivo assembly of nucleosomes |
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mcs5109 Wed, 12 Nov 2008 18:34:13 GMT |
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| Hsp90 |
-folds proteins involved in signal transduction |
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mcs5109 Wed, 12 Nov 2008 18:34:12 GMT |
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| Hsp70 |
-protein unfolding increased at high temps
-bind to newly synthesized proteins off ribosome
-help unfold proteins if they have to traverse a membrane |
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mcs5109 Wed, 12 Nov 2008 18:34:12 GMT |
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| dithiothreitol |
-oxidizes BPTI |
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mcs5109 Wed, 12 Nov 2008 18:20:04 GMT |
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| BPTI |
-bovine prancreatic trypsin inhibitor
-fully inactive if reduced
-3 disulfide bonds
-oxidized by dithiothreitol |
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mcs5109 Wed, 12 Nov 2008 18:20:04 GMT |
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| circular dichroism |
-measure amt of helical structure
-proteins absorb in UV region due to aromatic compounds |
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mcs5109 Wed, 12 Nov 2008 18:12:57 GMT |
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| intrinsic fluorscence of Rnase A |
-used to measure denaturing of protein |
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mcs5109 Wed, 12 Nov 2008 18:12:57 GMT |
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| Janus |
-similar to GB1 in structure
-shows not all aas important in protein structure |
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mcs5109 Wed, 12 Nov 2008 17:58:18 GMT |
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| GB1 |
-bacterial protein that binds to IgG
-change 50% of proteins to turn into Janus (Rop protein) |
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mcs5109 Wed, 12 Nov 2008 17:58:18 GMT |
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| mercaptoethanol |
-reduces disulfide bonds |
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mcs5109 Wed, 12 Nov 2008 17:42:59 GMT |
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| chaotropic salt |
-disrupts the three dimensional structure in macromolecules
-interfere with stabilizing intra-molecular interactions mediated by non-covalent forces such as hydrogen bonds, van der Waals forces, and hydrophobic effects. |
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mcs5109 Wed, 12 Nov 2008 17:42:59 GMT |
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| urea |
-chaotropic salt |
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mcs5109 Wed, 12 Nov 2008 17:42:59 GMT |
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| native vs. denatured energy chnage |
-small
-diff b/w entropy and enthalpy |
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mcs5109 Wed, 12 Nov 2008 17:42:59 GMT |
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| entropy |
-disorder
-favors denatured state |
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mcs5109 Wed, 12 Nov 2008 17:42:59 GMT |
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| enthalpy |
-energy of non-covalent bonds (H bonds, ionic bonds, etc.)
-favored in native state |
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mcs5109 Wed, 12 Nov 2008 17:42:59 GMT |
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