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| ooOOooOOooOOoo |
-eIF4F (at 5’ end of mRNA) associates with 3’ end of mRNA through poly-A binding protein
-when ribosome done, ideally positioned to restart translation on same mRNA
-recycling! |
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mcs5109 Thu, 23 Oct 2008 03:17:57 GMT |
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| scanning in euks |
-The small subunit moves 5’->3’ along the mRNA in an ATP-dependent process driven by eIF4F-associated RNA helicase
-finds AUG, some IFs released
-large subunit binds, all IFs released |
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mcs5109 Thu, 23 Oct 2008 03:15:59 GMT |
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| eIF4B |
-removes any secondary structure of mRNA by RNA helicase activity. |
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mcs5109 Thu, 23 Oct 2008 03:15:59 GMT |
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| eIF4F |
-recognizes 5’ cap structure of mRNA
-helps move small subunit along mRNA (w/ ATP) to find AUG
-associates with 3’ end of mRNA through poly-A binding protein |
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mcs5109 Thu, 23 Oct 2008 03:15:59 GMT |
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| translation initiation in euks |
>30 different polypeptides are involved in initiation of translation in eukaryotes |
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mcs5109 Thu, 23 Oct 2008 03:15:59 GMT |
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| after all 3 IFs bound, |
the small subunit is prepared to bind the mRNA and the initiator tRNA |
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mcs5109 Thu, 23 Oct 2008 03:00:58 GMT |
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| IF3 |
keep ribosomal subunits apart
(has to leave 1st so ribosome can work) |
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mcs5109 Thu, 23 Oct 2008 03:02:44 GMT |
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| IF2 |
with GTP, brings fMet-tRNA to the P site on the small subunit |
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mcs5109 Thu, 23 Oct 2008 03:00:58 GMT |
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| IF1 |
prevents tRNAs from binding to the portion of the small subunit that will become A site |
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mcs5109 Thu, 23 Oct 2008 03:00:58 GMT |
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| aminopeptidases |
remove the amino terminal methionine as well as one or two additional amino acids in pros |
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mcs5109 Thu, 23 Oct 2008 02:51:38 GMT |
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| Deformylase |
-removes the formyl group from the amino terminus during or after the synthesis of the polypeptide chain
-many pro proteins don't even have Met on N terminus |
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mcs5109 Thu, 23 Oct 2008 02:50:27 GMT |
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| initiator tRNA in pro |
N-formyl methionine |
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mcs5109 Thu, 23 Oct 2008 02:50:04 GMT |
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| SD equence base pairing |
(RBS, SD sequence) in the 5’ untranslated region and the 3’ end of the 16S rRNA |
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mcs5109 Thu, 23 Oct 2008 02:50:04 GMT |
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| initiation in pro mRNA |
-small subunit (30S) associates in mRNA
-base pairing b/w RBS and 16S rRNA
-AUG ideally in P site |
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mcs5109 Thu, 23 Oct 2008 02:50:04 GMT |
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