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| polypeptide exiting channel |
-only alpha helices can fit
-no B sheets
-other 2ary or 3ary have to form later |
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mcs5109 Thu, 23 Oct 2008 02:17:12 GMT |
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| mRNA binding channel |
-only ssRNA can fit
-removes 2ary structures |
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mcs5109 Thu, 23 Oct 2008 02:17:12 GMT |
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| kinky... |
...there's a kink in the mRNA site between A and P sites to facilitate maintenance of the correct reading frame |
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mcs5109 Thu, 23 Oct 2008 02:17:12 GMT |
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| E site |
binding site for the tRNA that is released after the growing polypeptide chain has been transferred to the aminoacyl-tRNA (E is for exit) |
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mcs5109 Thu, 23 Oct 2008 02:12:47 GMT |
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| P site |
binding site for peptidyl-tRNA |
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mcs5109 Thu, 23 Oct 2008 02:12:47 GMT |
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| A site |
binding site for aminoacylated-tRNA |
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mcs5109 Thu, 23 Oct 2008 02:12:47 GMT |
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| 16S rRNA |
-contacts anticodon loops
-decodes
-not some fancy-schmancy protein |
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mcs5109 Thu, 23 Oct 2008 02:09:59 GMT |
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| peptidyl transferase center |
-is composed entirely of RNA |
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mcs5109 Thu, 23 Oct 2008 02:09:59 GMT |
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| The bond b/w the ____ tRNA bond and growing peptide bond is broken |
peptidyl tRNA (P site) |
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mcs5109 Thu, 23 Oct 2008 02:06:40 GMT |
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| New Amino Acids Are Attached to the _______ of the Growing Polypeptide Chain |
-C terminus |
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mcs5109 Thu, 23 Oct 2008 02:05:11 GMT |
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| There is 1 ribosome for every ___ nucleotides of mRNA |
80 |
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mcs5109 Thu, 23 Oct 2008 02:05:11 GMT |
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| Polyribosome |
-mRNA bearing >1 ribosome
-if not, 90% of ribosomes just sittin' around doin' nuthin' |
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mcs5109 Thu, 23 Oct 2008 02:05:11 GMT |
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| Small subunit of ribosome consists of... |
...Decoding center: charged tRNAs read mRNA codons ("A")
-mRNA binding site |
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mcs5109 Thu, 23 Oct 2008 02:05:11 GMT |
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| Large subunit of ribosome consists of... |
...Peptidyl transfer center: forms peptide bonds ("P")
...Peptide exit channel ("E") |
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mcs5109 Thu, 23 Oct 2008 01:49:58 GMT |
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| What's % for ribosome? |
Ribosome consists of 2 subunits
60% RNA
40% protein |
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mcs5109 Thu, 23 Oct 2008 01:49:58 GMT |
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| What's the translation speed? |
-Translation speed of ribosome = 20 aa/sec (60 nt/sec)
RNA polymerase synthesizes 50-100 nt/sec |
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mcs5109 Thu, 23 Oct 2008 01:49:58 GMT |
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| in euks, do RNA polymerase and translation happen on same mRNA? |
-NO!!
-only in pros |
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mcs5109 Thu, 23 Oct 2008 01:49:58 GMT |
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