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| HO endonuclease |
introduces double-strand DNA break in MAT |
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mcs5109 Thu, 25 Sep 2008 16:55:50 GMT |
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| mitosis vs. meiosis |
Rad51 expresses both mitotically and meiotically, but Dmc1 is expressed in only meiosis |
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mcs5109 Thu, 25 Sep 2008 16:55:50 GMT |
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| Rad51 and Dmc1 |
are bacterial RecA homolog in eukaryote and play a role for meiotic recombination |
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mcs5109 Thu, 25 Sep 2008 16:35:17 GMT |
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| MRX complex |
(Mre11, Rad50 and Xrs2) replaces SPO11 protein on DNA to produce 3’ single stranded DNA (>1 kb) for homologous recombination |
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mcs5109 Thu, 25 Sep 2008 16:35:17 GMT |
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| Tyrosine side chain |
attacks the phosphate backbone to cut DNA, and generate covalent complex between protein and DNA |
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mcs5109 Thu, 25 Sep 2008 16:35:16 GMT |
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| SPO11: |
introduces double-strand breaks, cut-sites are located most commonly in chromosomal regions that are not tightly packed with nucleosomes such as promoters (hotspots for recombination) |
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mcs5109 Thu, 25 Sep 2008 16:35:16 GMT |
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| RuvC (dimer) is |
DNA endonuclease to resolve Holiday junction
RuvC cleaves DNA with modest sequence specificity. The consensus sequence is found frequently in DNA, but the sequence specificity ensures at least some branch migration occurs before resolution |
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mcs5109 Thu, 25 Sep 2008 16:19:27 GMT |
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| RuvB |
ATP-dependent hexameric DNA helicase for branch migration |
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mcs5109 Thu, 25 Sep 2008 16:19:27 GMT |
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| RuvA |
Holiday junction specific DNA binding protein, recruiting RuvB proteins |
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mcs5109 Thu, 25 Sep 2008 16:19:27 GMT |
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| RecA Homologs Are Present in All Organisms |
RecA: eubacteria
RadA: archaeal
Rad51 and Dmc1: eukaryote
UvsX: T4 phage
These homologs form similar filament structures |
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mcs5109 Thu, 25 Sep 2008 16:19:27 GMT |
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| RuvC (E. coli) |
resolution of Holiday junctions |
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mcs5109 Thu, 25 Sep 2008 15:49:58 GMT |
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| RuvAB (E. coli) |
Holiday junction recognition and branch migration |
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mcs5109 Thu, 25 Sep 2008 15:49:58 GMT |
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| RecBCD and RecFOR (E. coli) |
assembly of strand-exchange proteins |
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mcs5109 Thu, 25 Sep 2008 15:49:58 GMT |
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| RecBCD helicase/nuclease (E. coli) |
processing DNA breaks to generate single strands for invasion |
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mcs5109 Thu, 25 Sep 2008 15:49:58 GMT |
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| None (E. coli) |
introduction of DSB
-breaks introduced via DNA damage or failure of rep fork |
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mcs5109 Thu, 25 Sep 2008 15:54:34 GMT |
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| RecA (E. coli) |
pairing homologous DNA and strand invasion |
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mcs5109 Thu, 25 Sep 2008 15:49:58 GMT |
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