BMB 400 Chapter 8 Flash Cards

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DNA helicases	-Heximeric proteins -ATP -Ring shaped protein complexes encircle the DNA strand (increase processivity) -moves directionally	0	mcs5109 Thu, 25 Sep 2008 05:57:19 GMT
5’ exonuclease	-removes final RNA that removed from 5' end	0	mcs5109 Thu, 25 Sep 2008 05:57:19 GMT
RNA primers are removed by	1) RNase H plus 5’ exonuclease or 2) 5’->3’ exonuclease activity of DNA pol I DNA pol I fills gap and DNA ligase repairs a nick	0	mcs5109 Thu, 25 Sep 2008 05:57:19 GMT
RNA primer	(5~10 nt long)	0	mcs5109 Thu, 25 Sep 2008 03:14:48 GMT
Primase	-RNA primer (5~10 nt long) -does not require specific DNA sequences to initiate syntheis -activated when associated with DNA helicase -removed by RNase H plus 5’ or 5’->3’ exonuclease activity of DNA pol I	0	mcs5109 Thu, 25 Sep 2008 03:14:48 GMT
Processivity	-average number of nucleotides added each time the enzyme binds a primer:template junction	0	mcs5109 Thu, 25 Sep 2008 03:14:48 GMT
Kinetic selectivity	-Incorrect nucleotide incorporation is as much as 10,000-fold slower than incorporation when base pairing correct. Error rate ~10-5	0	mcs5109 Thu, 25 Sep 2008 03:14:47 GMT

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